Figure 4.

Photomicrographs of PicroSirius red staining. (A) Corticoid-treated and controls’ wound area showed a black area (especially on the surface), indicating the absence or a small number of collagen fibers. On the side and bottom of the wound, poorly organized thin collagen fibers were observed. Copaiba-treated wounds were filled with more organized collagen fibers. (PSR, original magnification, ×100 and ×400). (B) Graphs of collagen content analysis on D3 (n = 15) and (C) D7 (n = 15). Note that on D3 and D7, corticoid-treated and control groups showed less organized fibers than the copaiba-treated group (original magnification ×100 and ×400). One-way ANOVA followed by Tukey’s multiple comparison tests (* p < 0.05, ** p < 0.01).