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. 2020 May 15;21(10):3503. doi: 10.3390/ijms21103503

Table 1.

Oligonucleotide primer sequences used for quantitative reverse transcription-polymerase chain reaction.

Gene Primer Sequence (Forward) Primer Sequence (Reverse) Annealing Temperature (°C) Product Size (bp) RefSeq Number
CYP1A1 5’- CATCCCCCACAGCACCATAA -3’ 5’- TTCGCTTGCCCAAACCAAAG -3’ 60 212 NM_012540.2
CYP1B1 5’- TGCTACTCGTTTCGGTCCTG -3’ 5’- CAAGGCGAGCGAAGTACAAG -3’ 60 162 NM_012940.2
iNOS 5’- AGGCCACCTCGGATATCTCT -3’ 5’- TCTCTGGGTCCTCTGGTCAA -3’ 60 85 NM_012611.3
NFκB 5’- TGTCTGCACCTGTTCCAAAGA-3’ 5’- TGCCAGGTCTGTGAACACTC-3’ 60 143 NM_199267.2
IL6 5’- AGAGACTTCCAGCCAGTTGC-3’ 5’- TGAAGTCTCCTCTCCGGACT-3’ 60 88 NM_012589.2
TNFα 5’- CGTCAGCCGATTTGCCATTT -3’ 5’- TCCCTCAGGGGTGTCCTTAG -3’ 60 88 NM_012675.3