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. 2020 May 16;21(10):3537. doi: 10.3390/ijms21103537

Figure 2.

Figure 2

Melanin synthesis in cultured human epidermal melanocytes after 140 h exposure to the indicated treatments with 6-SG. (A) Photography of cultured cells with medium; (B) Melanin content quantification by melanin content assay in culture medium; (C) Melanin content quantification by melanin content assay in cell lysates; (D) Tyrosinase and (E) MITF) mRNA expression level evaluation by real-time PCR analyses with normalization to that of GAPDH. Data (B, C, D and E) represent the results of three independent experiments. Data are shown as mean ± SD. ***, p < 0.01.