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. 2020 May 15;21(10):3491. doi: 10.3390/ijms21103491

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Expression of germ cell markers in 30-day cultured mouse testicular fragments (MTFs). The mRNA levels of the germ cell markers (A) Sycp3, (B) Vasa, (C) Dazl, (D) Sohlh1, (E) Sohlh2, (F) Zbtb16, and (G) Gfra1 in the MTFs were determined using quantitative polymerase chain reaction. The relative quantification of mRNA is shown using the mean and the standard error of the mean (n = 6) at log₂ scale. The levels of undifferentiated and differentiated germ cell markers distinctly decreased in a dose-dependent manner in 30-day cultured MTFs with nonylphenol (NP).