Figure 4.

Representative Picrosirius Red stained pulmonary specimens (a–f) in healthy control (a), M. U-care–treated mice (b,c), and untreated (d–f) mice. A widespread occurrence of collagen fibrils bundles radiating from bronchiolar wall to the lung parenchyma, also inside the tumours masses, are observable (b,d,e). Collagen fibers encircling the metastases outline are also shown (c, f). Light microscopy magnification: 40× (a–f); 60× (insert in d). Histograms showing the quantitative analysis of collagen fibers OD (H) and bronchiolar wall area (I). p value calculated using one-way ANOVA followed by Bonferroni’s post-hoc test: (***) < 0.001.