Figure 5.

Immunohistochemical labelling for IL-6 in healthy control (a), Mic U-care–treated (b, c), and untreated (d–g) mice. While a weaker immunoreactivity is shown in M. U-care–treated mice, the heaviest IL-6-immunopositivity is observable in untreated mice in alveolar pneumocytes, in metastatic cells as well as in bronchiolar epithelial cells (b–g), evidenced by the presence of several immunopositive endothelial cells (d,f). IL-6 antigen appeared particularly overexpressed in metastatic tissue (e,g). Light microscopy magnification: 40× (a–c,f,g); 60× (d,e). Histograms showing the quantitative analysis of immunopositive cell density (H) and OD (I). p values calculated by one-way ANOVA followed by Bonferroni’s post-hoc test and unpaired Student’s t-test for parenchyma and metastases, respectively: (*) < 0.05; (**) < 0.01.