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. 2020 May 15;21(10):3519. doi: 10.3390/ijms21103519

Figure 5.

Figure 5

L-4F inhibits M1-macrophage activation and induces macrophage plasticity in vitro. (A) RT-PCR analysis of M1 and M2 macrophage-associated marker genes in mouse peritoneal macrophages polarized in vitro. (B) RT-PCR analysis of M1 and M2 macrophage-associated marker genes from polarized RAW 264.7 cells (upper panels) or polarized mouse peritoneal macrophages (lower panels) after overnight treatment with either PBS or L-4F (50 μg/mL). Data are presented as fold changes (mean ± SD) and compared to non-polarized M0 macrophages. * p < 0.05 vs. respective M0; $ p < 0.05 vs. PBS-treated M1 group, @ p < 0.05 vs. respective M1 and M2 groups, n = 3/group each done in triplicate for RAW 264.7 cells. Thioglycolate-elicited mouse peritoneal macrophages were isolated and pooled from 6 mice and also run in triplicate.