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. 2020 May 17;21(10):3547. doi: 10.3390/ijms21103547

Figure 2.

Figure 2

RORγt-GFP+ cells in the retinal vasculature (A) Representative fluorescent microscopy images of RORγt/GFP+ cells in retinas of non-diabetic and diabetic RORγt-GFP reporter mice (n = 3/group) following perfusion and vascular staining (scale bars of all images = 25 μm, which is a visual indicator of the size of the representative image). (B) Representative flow cytometry overlay of RORγt/GFP+ cells in the retina vasculature of non-diabetic (black) and STZ-induced diabetic (red) RORγt-GFP mice. (C) Flow cytometry quantification (n = 5 pooled samples (15 mice)/group) of percent positive (of 10,000 events) RORγt/GFP+ cells in retinas of non-diabetic (white) and diabetic (black) RORγt-GFP mice. Error bars represent the SEM, and * p < 0.01 per unpaired student’s t-test. All data was collected 2 months after diabetes was confirmed. Data are representative of two separate experiments with similar results.