Figure 5.

Leukostasis in the retinal vasculature of SR1001 treated diabetic mice. (A) Representative fluorescent microscopy images (scale bars of all images = 100 μm, which is a visual indicator of the size of the images) of leukocytes adhered to the retinal vasculature (highlighted by red arrows) in non-diabetic, diabetic, and SR1001 treated diabetic C57BL/6 mice following perfusion and vascular staining. Lower images are an individual retina vessel magnified 40× with adherent leukocytes highlighted by red arrows. (B) Quantification of adherent leukocytes to the vasculature in the retinas (n = 3) of 3 mice/group (B). Error bars represent the SEM, and * p < 0.01, wherein p-value was first equated by two-way ANOVA analysis and then an unpaired t-test with Tukey’s post-hoc analysis.