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. 2020 May 19;21(10):3583. doi: 10.3390/ijms21103583

Figure 4.

Figure 4

Cilastatin preconditioning protected IR-induced cell death via the activation of HIF-1α in HK-2 cells. (A) Semiquantitative immunoblotting of HIF-1α expression revealed that IR injury upregulated HIF-1α expression and cilastatin pretreatment further enhanced HIF-1α expression in IR-exposed cells. Cilastatin treatment also prevented IR-induced cell death. Co-treatment with cilastatin and (B) HIF-1α inhibitor, YC-1, or (C) HIF-1α siRNA restored cell death similar to those of IR-exposed cells without cilastatin treatment. The data are presented as means ± SEM. * p < 0.05.