Table 1.
Renal cell strains were exposed to 0, 1, 10, 100 and 1000 nM AF for 24 h in Dulbecco’s modified Eagle’s medium containing 4.5 gm/L D-glucose supplemented with 5% fetal calf serum. Cells were processed, and CYP1A1 and CYP1B1 mRNA was quantitated by RT-PCR. Fold CYP1A1 or CYP1B1 mRNA induction relative to the untreated controls is indicated regarding the AF concentration for which induction was observed. Cells with high apoptosis are shown (data reported in [33]).
| AF nM Concentration (Fold mRNA Induction) | ||||
|---|---|---|---|---|
| AF resistant | Type | CYP1A1 mRNA | CYP1B1 mRNA | Highest apoptosis |
| 115 | Clear | None | None | |
| 121 | Clear | 100 (4), 1000 (82) | None | |
| 150 | Clear | None | None | |
| 151 | Clear | None | None | |
| 154 | Clear | 100 (2) | None | |
| 171 | Clear | 100 (2), 1000 (10) | 100 (2); 1000 (7) | |
| 181 | Clear | None | None | |
| 124 | Papillary | 1, 10, 100, 1000 (2) | None | |
| AF sensitive | ||||
| 109 | Clear | None | 1 (4); 10 (2) | x |
| 117 | Clear | 100 (2), 1000 (7) | 100 (2); 1000 (5) | |
| 112 | Papillary | 10 (2), 100 (11), 1000 (27) | 1, 10 (2); 100 (3); 1000 (7) | x |
| 132 | Papillary | 1, 100, 1000 (2), 10 (9) | 1000 (2) | x |
| 146 | Papillary | 1, 10, 100, 1000 (2) | 1 (8); 10 (27); 100 (39); 1000 (27) | x |