Figure A3.

Heatmap of ERAD (A) and autophagy (B) proteins identified, using mass spectrometry for the indicated sucrose gradient fractions that were immunoprecipitated with EDEM1; the eluted samples were separated by SDS-PAGE, followed by in the gel digestion protocol, as described in the Materials and Methods section. Identified proteins were annotated with GO terms from UniProt database, and entries containing the ERAD (A) and autophagy (B) key-terms were further analysed. Positive values, after negative control subtraction of spectral counts in log scale corresponding to each extraction condition, are represented as a heatmap.