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. 2020 May 18;21(10):3576. doi: 10.3390/ijms21103576

Figure 3.

Figure 3

The effect of prenatal exposure to VPA on the expression of v-SNARE proteins: VAMP1/2, Syp, Syn1, p-Syn1(Ser62/Ser67), and Syt1. The level of mRNA of Vamp1, Vamp2, Syp, Syn1, and Syt1 in the hippocampus (A) and cerebral cortex (C) of the control and VPA-exposed rats is presented. The level of mRNA was measured by real-time PCR and calculated by the ΔΔCt method with Actb (β-actin) as a reference gene. The data represent the mean values ± SEM from n = (4–5) independent experiments in the hippocampus and n = (4–6) in the cerebral cortex. The immunoreactivity of the v-SNARE proteins in the control and the VPA-exposed rats was monitored using a Western blot analysis. Densitometric analysis and representative pictures of VAMP1/2, Syp, Syn1, p-Syn1, and Syt1 in the hippocampus (B) and cerebral cortex (D) are shown. Results were normalized to GAPDH levels. Data represent the mean values ± SEM from n = (3–9) independent experiments in both the hippocampus and cerebral cortex. * p < 0.5, ** p < 0.01, *** p < 0.001 vs. control.