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. 2020 May 18;21(10):3576. doi: 10.3390/ijms21103576

Figure 4.

Figure 4

The effect of prenatal exposure to VPA on the expression of the t-SNARE proteins: SNAP25 and Stx1. The gene expression of Snap25, Stx1a, and Stx1b in the hippocampus (A) and cerebral cortex (C) of the control and VPA-exposed rats was measured via quantitative RT-PCR and calculated by the ΔΔCt method with Actb (β-actin) as a reference gene. Data represent the mean values ± SEM from n = (4–6) independent experiments in both the hippocampus and cerebral cortex. The immunoreactivity of the t-SNARE proteins in the control and the VPA-exposed rats was monitored using a Western blot analysis. Representative pictures and a densitometric analysis of SNAP25 and syntaxin 1 (Stx1) in the hippocampus (B) and cerebral cortex (D) are presented. The results were normalized to GAPDH levels. The data represent the mean values ± SEM from n = 5 independent experiments in both the hippocampus and cerebral cortex. ** p < 0.01, vs. control.