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. 2020 May 18;21(10):3576. doi: 10.3390/ijms21103576

Figure 6.

Figure 6

The effect of prenatal exposure to VPA on the expression of the synaptic cell adhesion molecules: Nlgn1 and Nlgn3. The gene expression of Nlgn1 and Nlgn3 in the hippocampus (A) and cerebral cortex (C) of the control and VPA-exposed rats was measured with quantitative RT-PCR and calculated by the ΔΔCt method with Actb (β-actin) as a reference gene. The data represent the mean values ± SEM from n = (5–6) independent experiments in both the hippocampus and cerebral cortex. The immunoreactivity of Nlgn1 and Nlgn3 in the control and VPA-exposed brains was monitored using a Western blot analysis. Densitometric analysis and representative pictures for Nlgn1 and Nlgn3 in the hippocampus (B) and cerebral cortex (D) are shown. Results were normalized to vinculin levels. Data represent the mean values ± SEM from n = (11–14) independent experiments in both the hippocampus and cerebral cortex. * p < 0.5, ** p < 0.01 vs. control.