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. 2020 May 18;21(10):3576. doi: 10.3390/ijms21103576

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Experimental design. (A) Pregnant Wistar rats were injected at gestation day 12.5 (GD 12.5) with NaCl or VPA (450 mg/kg b.w.). One group of offspring rats (males and females) was subjected to an ISO test at postnatal day 11 (PND 11) and then to an Open-field test (males only) at PND 55. Other groups of offspring rats (males) was subjected to Open-field test at PND 55 and then to a 3-chamber test at PND 57. All offspring rats were euthanized at PND 58; tissue samples from the hippocampus and cerebral cortex were collected and used for biochemical, immunochemical, genetic, and microscopic analysis. (B) Design of the 3-chamber test. A detailed description is included in the behavioural analysis section.