Figure 2.

A concomitant increase of mitochondria fusion and fission markers during TA remobilization. Protein levels for FIS1, MFN2, and OPA1 were assessed by Western blots in the GA (A) and the TA (B), quantified and normalized using Ponceau red staining for uneven loading. Several isoforms of OPA1 tune mitochondrial adaptations. Thus, the 2 isoforms detected here were quantified together. Representative Western blots are shown below each graph, and molecular weights are given in kDa. Data were expressed as % from the Con group. Statistical differences were assessed by ANOVA, as described in Materials and Methods. * p < 0.05 vs. Con, ^§ p < 0.05 vs. Imm; Con, non-immobilized rats; Imm, immobilized; Rem, remobilized.