Figure 5.

Autophagy was induced during muscle immobilization and remobilization. Protein levels for SQSTM1, LC3I, and LC3II were assessed in GA (A,B) and TA (A,C) muscles by Western blots. Two forms of LC3 were detected (LC3-I and LC3-II) and were quantified separately. Signals were quantified and normalized using Ponceau red staining for uneven loading. Representative Western blots are shown in (A), and molecular weights are given in kDa. Data were expressed as % from the Con group. Statistical differences were assessed by ANOVA, as described in Materials and Methods. * p < 0.05 vs. Con, ^§ p < 0.05 vs. Imm; Con, non-immobilized rats; Imm, immobilized; Rem, remobilized.