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. 2020 May 25;21(10):3724. doi: 10.3390/ijms21103724

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) Images of multinucleated giant cells (MNGCs) stained with 10% (v/v) Giemsa after 10 days cultivation on poly (ethylene imine) (PEI) and terminal layers of PEMs composed of either hyaluronic acid (HA) or heparin (Hep) as polyanions and chitosan (Chi) as polycation abbreviated as (PEI(HA/Chi)₄HA, PEI(Hep/Chi)₄Hep), respectively. Scale bar: 100 μm. (B) The area percentage of MNGCs on PEI, PEI(HA/Chi)₄HA and PEI(Hep/Chi)₄Hep surfaces based on quantitative image analysis of micrographs. Results represent means ± SD, * p < 0.05, n = 15.