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. 2020 May 25;21(10):3724. doi: 10.3390/ijms21103724

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) Representative confocal laser microscopy (CLSM) images of adherent macrophages stained with the membrane stain DID (red colour) after 24 h cultivation on poly (ethylene imine) (PEI) and terminal layers of PEMs composed of either hyaluronic acid (HA) or heparin (Hep) as polyanions (stained with FITC, green colour) and chitosan (Chi) as a polycation abbreviated as (PEI(HA/Chi)₄HA, PEI(Hep/Chi)₄Hep), respectively (63-fold oil immersion objective, Scale bar: 10 µm). (B) Representative 3D view of a z-stacks in surface projection with CLSM (63-fold oil immersion objective, scale: 20 μm). In this mode, pixel values are computed as solids, which allows no transparency.