Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 May 12;21(10):3428. doi: 10.3390/ijms21103428

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Targeted protein levels in rat fetal brain. The levels of fetal brain targeted proteins were detected via Western blotting and normalized using Ponceau S staining. (A) Representative band densities are shown. Relative abundance of (B) pPPARγ/PPARγ, (C) pIRS1 (s616)/IRS1, (D) pIRS2 (s731)/IRS2, (E) pAKT/AKT, (F) adiponectin, (G) SIRT1, and (H) BDNF were quantified. One-way ANOVA followed by Fisher’s LSD post hoc was used to assess the significant differences among groups and multiple comparisons. pPPARγ/PPARγ: (F (3,20) = 3.470, p < 0.05). pIRS2 (s731)/IRS2: (F (3,24) = 3.647, p < 0.05). pAKT/AKT: (F (3,20) = 4.261, p < 0.05). Adiponectin: (F (3,20) = 4.226, p < 0.05). SIRT1: (F (3,20) = 5.828, p < 0.01). BDNF: (F (3,20) = 3.445, p < 0.05). n = 10–12 for each group. * p < 0.05 vs. C; ** p < 0.01 vs. C; # p < 0.05 vs. H; ## p < 0.01 vs. H; PPARγ: peroxisome proliferator-activated receptors γ; IRS: insulin receptor substrate; AKT: alpha serine/threonine-protein kinase; SIRT1: sirtuin 1; BDNF: brain-derived neurotrophic factor.

Targeted protein levels in rat fetal brain. The levels of fetal brain targeted proteins were detected via Western blotting and normalized using Ponceau S staining. (A) Representative band densities are shown. Relative abundance of (B) pPPARγ/PPARγ, (C) pIRS1 (s616)/IRS1, (D) pIRS2 (s731)/IRS2, (E) pAKT/AKT, (F) adiponectin, (G) SIRT1, and (H) BDNF were quantified. One-way ANOVA followed by Fisher’s LSD post hoc was used to assess the significant differences among groups and multiple comparisons. pPPARγ/PPARγ: (F (3,20) = 3.470, p < 0.05). pIRS2 (s731)/IRS2: (F (3,24) = 3.647, p < 0.05). pAKT/AKT: (F (3,20) = 4.261, p < 0.05). Adiponectin: (F (3,20) = 4.226, p < 0.05). SIRT1: (F (3,20) = 5.828, p < 0.01). BDNF: (F (3,20) = 3.445, p < 0.05). n = 10–12 for each group. * p < 0.05 vs. C; ** p < 0.01 vs. C; # p < 0.05 vs. H; ## p < 0.01 vs. H; PPARγ: peroxisome proliferator-activated receptors γ; IRS: insulin receptor substrate; AKT: alpha serine/threonine-protein kinase; SIRT1: sirtuin 1; BDNF: brain-derived neurotrophic factor.