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. 2020 May 12;21(10):3428. doi: 10.3390/ijms21103428

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The experimental design and timeline. Approximately 8–9-week-old female rats were fed a chow diet or HFD for 8 weeks. Placenta and fetal brains were collected for cohort 1 study. After mating and delivery of pups, male baby rats were weaned and fed a chow diet or a HFD from PND 21 onwards for cohort 2 study. Cohort 1 included four experimental groups (n = 12–14 per group). C: maternal rat chow; CR: maternal rat chow diet + maternal resveratrol; H: maternal HFD/obesity; HR: maternal HFD/obesity + maternal resveratrol. Cohort 2 included five experimental groups (n = 12–14 per group). CC: maternal rat chow diet + postnatal chow diet; HC: maternal HFD/obesity + postnatal chow diet; CH: maternal rat chow diet + postnatal HFD; HH: maternal/HFD/obesity + postnatal HFD; HRH: maternal HFD/obesity + maternal resveratrol + postnatal HFD.