Figure 1.

An example for the growth of the wild-type and the mutant L. corymbifera strains on yeast nitrogen base (YNB) medium (a), YNB medium supplemented with uracil (0.5 g/L) (b) and YNB medium supplemented with uracil (0.5 g/L) and 5-FOA (1 g/L) (c). Strains: CBS 429.75, the original wild-type strain; CBS 429.75–pyrG^Cr1/1, one of the mutants, in which the pyrG was disrupted by the CRISPR-Cas9 method; CBS 429.75–pyrG^compl/1, the strain, in which the CRISPR-Cas9-generated mutation was complemented. Growth was performed at 37 °C for two days.