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. 2020 May 25;21(10):3727. doi: 10.3390/ijms21103727

Figure 2.

Figure 2

CRISPR-Cas9 induces deletions in the targeted sites of the L. corymbifera pyrG gene. (a) Sequence of the site in the pyrG gene targeted using the LcpyrGcr1 protospacer and location of the induced mutations in the three resulting strains. (b) Sequence of the site in the pyrG gene targeted using the LcpyrGcr2 protospacer and location of the induced mutations in the seven resulting strains. The protospacer and the protospacer adjacent motif (PAM) sequence are highlighted with blue and red letters, respectively. (c) Diagram representing the pyrG gene and showing the positions of the targeted sites and the primers used to analyze the mutants.