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. 2020 May 25;21(10):3727. doi: 10.3390/ijms21103727
5-FOA 5-fluoroorotic acid
Cas9 CRISPR-associated protein 9
CFW calcofluor white
CRISPR clustered regularly interspaced short palindromic repeats
crRNA CRISPR-RNA
gRNA guide RNA
H2O2 hydrogen peroxide
HDR Homology-directed repair
MEA malt extract agar
NHEJ non-homologous end-joining
OMP orotidine 5′-phosphate
PAM protospacer adjacent motif
PBS phosphate buffer saline
PEG polyethylene glycol
pyrF orotate phosphoribosyl transferase gene
pyrG orotidine 5′-phosphate decarboxylase gene
SDS sodium dodecyl sulfate
tracrRNA trans-activating crRNA
YNB yeast nitrogen base