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. 2020 May 13;21(10):3443. doi: 10.3390/ijms21103443

Figure 1.

Figure 1

BBR reduced the ARpolyQ protein level. (A) Representative confocal microscopy analysis of GFP-AR.Q(n) intracellular localization. NSC34 cells were transfected with GFP-ARQ.22 or GFP-AR.Q48 in absence or in presence of 10 nM testosterone. Nuclei were stained with DAPI (63X magnification). Scale bar: 10 μM. (B) WB analysis performed on NSC34 cells transfected with AR.Q46 in absence or in presence of 10 nM testosterone, untransfected control was added to test the antibody specificity. GAPDH was used as loading control. (C) MTT cell viability assay was performed on NSC34 cells treated with BBR at different concentration for 48 h (* p < 0.05, *** p < 0.001, one-way ANOVA, followed by Tukey’s test). (D,E) NSC34 cells were transfected with AR.Q46 in absence or presence of 10 nM testosterone and BBR at three different doses (0.05, 0.1, and 0.2 μM for 48 h Ethanol and DMSO were used as vehicle control for testosterone and BBR, respectively. (D) WB analysis was performed. GAPDH was used as loading control, and the bar graph represents the mean optical density ± SD of AR: GAPDH (n = 3) (E) FRA was performed, the bar graph represents the mean optical density of AR ± SD (n = 3). (* p < 0.05, ** p < 0.01, *** p < 0.001, two-way ANOVA, followed by Tukey’s test).