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. 2020 May 21;21(10):3653. doi: 10.3390/ijms21103653

Figure 2.

Figure 2

miRNA silencing limits the effect of HP on miR-34a, miR-146a, and miR-181a. (A–D) Expression levels of miR-34a, miR-146a, and miR-181a analyzed by real-time PCR. Human OA chondrocytes were evaluated at basal condition, after 24~h of transfection with miR-34a, miR-146a, and miR-181a inhibitors (50 nM) or NC (5 nM), and after 3~h of low sinusoidal (1–5 MPa) or static continuous (10 MPa) HP exposure. The gene expression was referenced to the ratio of the value of interest and the value of basal condition, reported equal to 1. Data were expressed as mean ± SD of triplicate values. * p < 0.05, ** p < 0.01 versus basal condition or NC. ° p < 0.05 versus inhibitor. # p < 0.05 versus HP. INIB = Inhibitor, NC = negative control siRNA.