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. 2020 May 21;21(10):3653. doi: 10.3390/ijms21103653

Figure 5.

Figure 5

miRNA inhibition mediates HP effect on apoptosis. (A–D) Apoptosis detection performed by flow cytometry analysis and measured with Annexin Alexa fluor 488 assay. Data were expressed as the percentage of positive cells for Annexin-V and propidium iodide (PI) staining. (E–H) Expression levels of BCL2 analyzed by real-time PCR. Human OA chondrocytes were evaluated at basal condition, after 24~h of transfection with miR-34a, miR-146a, and miR-181a inhibitors (50 nM) or NC (5 nM), and after 3~h of low sinusoidal (1–5 MPa) or static continuous (10 MPa) HP exposure. The ratio of apoptosis and the gene expression were referenced to the ratio of the value of interest and the value of basal condition or NC, reported equal to 1. Data were expressed as mean ± SD of triplicate values. * p < 0.05, ** p < 0.01 versus basal condition or NC. ° p < 0.05 versus inhibitor. # p < 0.05, ## p < 0.01 versus HP. INIB = Inhibitor, NC = negative control siRNA.