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. 2020 May 21;21(10):3653. doi: 10.3390/ijms21103653

Figure 6.

Figure 6

miRNA silencing regulates oxidative stress following HP exposure. (A–D) Mitochondrial superoxide anion production evaluated by MitoSox Red staining at flow cytometry. Human OA chondrocytes were evaluated at basal condition, after 24~h of transfection with miR-34a, miR-146a, and miR-181a inhibitors (50 nM) or NC (5 nM), and after 3~h of low sinusoidal (1–5 MPa) or static continuous (10 MPa) HP exposure. The production of superoxide anion was referenced to the ratio of the value of interest and the value of basal condition or NC, reported equal to 1. Data were expressed as mean ± SD of triplicate values. * p < 0.05, ** p < 0.01 versus basal condition or NC. ° p < 0.05 versus inhibitor. # p < 0.05 versus HP. INIB = Inhibitor, NC = negative control siRNA.