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. 2020 May 21;21(10):3653. doi: 10.3390/ijms21103653

Figure 9.

Figure 9

miR-34a inhibition mediates HP effect on β-catenin protein expression. Immunofluorescence labeling of β-catenin localization. Human OA chondrocytes were evaluated at basal condition, after 24~h of transfection with miR-34a inhibitor, and after 3~h of low sinusoidal (1–5 MPa) or static continuous (10 MPa) HP exposure. (A) Representative immunocytochemical images of the cells showing localization of β-catenin (red); nuclei were stained with DAPI (blue). Original magnification 400×. Scale bar: 20 µm. (B) The histogram of immunolabeling intensity was plotted for the nuclear and cytoplasm expression for β-catenin. Data were expressed as mean ± SD of triplicate values. * p < 0.05, ** p < 0.01, *** p < 0.001 versus basal condition. ° p < 0.05 versus inhibitor. # p < 0.05 versus HP. INIB = Inhibitor.