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. 2020 May 20;21(10):3610. doi: 10.3390/ijms21103610

Figure 3.

Figure 3

Effect of miR-370 restoration in GSCs. (A) Pathway enrichment analysis examined by GSEA software of highly modulated genes (≥2 fold both up and down) in GSC#1 transduced with TRIPZ-miR-370 vs TRIPZ empty vector. (B) RT-PCR analysis performed on GSC#1 and GSC#83 transduced with TRIPZ or TRIPZ-miR-370 for HMGA2 (left panel) and Hypoxia-inducible factor (HIF) 1A expression (right panel). * p < 0.05; ** p < 0.01. (C) Predicted binding site of miR-370-3p in the HIF1A 3′UTR sequence (left panel). Dual-luciferase reporter assay of pGL3 vectors containing the HIF1A 3′UTR or HIF1A mutant (mut), co-transfected in 293T cells with hsa-miR-370-3p-mimic or control mimic RNA (ctrl). ** p < 0.01. Histograms show normalized mean values of the relative luciferase activity. Error bars represent the mean ± SD (n = 4) (right panel) (D) FACS analysis of NT5E/CD73 on GSC#1 and GSC#83 transduced with TRIPZ empty vector and TRIPZ-miR-370 after doxycycline induction.