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Comparison between 2D and 3D cell culture assays.
| Parameter |
2D Assays |
3D Assays | ||
|---|---|---|---|---|
| Organoids | Decellularized ECM | Bioprinted Constructs | ||
| Cell-to-Cell, Cell-to-ECM Interactions | Poor | Excellent | Excellent | Excellent |
| Tissue microenvironment | Extremely Limited | Limited | Good Duplication | Limited |
| Control of structural architecture | No | No | Yes, but influenced by chemical and physical preparations | Yes |
| Presence of connective tissue cells | Possible but not used | Yes | Yes | Yes |
