Figure 3.

Analysis of the product (cut spectrin) of calcium-dependent calpain1 enzymatic activity. (a) Immunoblotting probed with antibody specific for spectrin on neuronal precursor cells (NPCs) and (b, top panel) on iPS derived-neurons (20 µg of total proteins). The asterisks indicate non-specific bands. (b, lower panel) Immunoblotting probed with antibody specific for calpain1 on iPS derived-neurons. β-actin was used as loading control in all the immunoblotting. Graph of the ratio of cut spectrin/total spectrin in neuronal precursor cells and in iPS derived-neurons and of calpain1/β-actin in iPS derived-neurons are shown on the right side of the respective blotting. The statistical analysis was conducted using student-t-test: * p < 0.05, ** p < 0.01. All data are presented as mean + SEM on at least three independent replicates.