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. 2020 May 23;21(10):3678. doi: 10.3390/ijms21103678

Figure 3.

Figure 3

Inhibitory effect of LMDIR on neuroinflammation in the subiculum and cerebral cortex of 5XFAD mice. (A) Representative images of immunofluorescent staining of the subiculum and cerebral cortex for Iba-1, a marker of microglia. (B) Representative images of immunofluorescent staining of the subiculum and cerebral cortex for GFAP, a marker of astrocytes. (C,D) The number of Iba-1 (+) cells was significantly higher in the subiculum and cerebral cortex of 5XFAD mice than that of the WT mice. In contrast, the number of Iba-1 (+) cells was significantly lower in the subiculum and cerebral cortex of LMDIR-exposed 5XFAD mice than that of the sham-exposed 5XFAD mice. (E,F) The number of GFAP (+) cells was significantly higher in the subiculum and cerebral cortex of 5XFAD mice than the WT mice. However, the number of GFAP (+) cells was significantly lower in the subiculum and cerebral cortex of LMDIR-exposed 5XFAD mice than that of the sham-exposed 5XFAD mice. Data are presented as mean ± SEM (n = 7 in each group). ### p < 0.001: WT mice versus sham-exposed 5XFAD mice. *** p < 0.001: sham-exposed 5XFAD mice versus LMDIR-exposed 5XFAD mice. Scale bar = 50 and 100 μm (Sub and Ctx) or 500 μm (hemisphere). LMDIR, low–moderate dose ionizing radiation; Iba-1, ionized calcium-binding adaptor molecule 1; GFAP, glial fibrillary acidic protein.