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Tubular damage and cell death at 24 h in renal IRI and sham controls for WT (closed circles) and CypA−/− (open circles) mice. RT-PCR for mRNA levels of (A) KIM1, and (B) Kotho. (C) Quantification of the number of TUNEL+ tubular cells. (D) A dose-response of H2O2 induced cell death in primary cultures of tubular epithelial cells from WT and CypA−/− mice. Data are mean ± SD. * p < 0.05, *** p < 0.0001 versus WT sham control.
