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Neutrophil and macrophage infiltration at 24 h in renal IRI and sham controls for WT (closed circles) and CypA−/− (open circles) mice. (A) Immunoperoxidase staining for infiltrating neutrophils. Bar = 100 μm. (B) Quantification of neutrophil infiltration. RT-PCR analysis of (C) CD68, and (D) CD3 mRNA levels. Data are mean ± SD. ** p < 0.05, *** p < 0.0001 versus WT sham control. NS, not significant.
