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. 2020 May 14;10(5):942. doi: 10.3390/nano10050942

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) Sketch of the anisotropic directional building blocks (AuNRs@T₁₈-DNA@Ag_50min, top) and their anisotropic directional nanoassemblies (bottom). The orange shading highlights the spots of highest electric-field enhancement, i.e., the strategically positioned hot-spots that can lead to SERS of the ssDNA (top panel) or of the dsDNA (bottom panel). (B) Representative SERS spectra of a single AuNRs@T₁₈-DNA@Ag (directional building block, black curve) and of a single anisotropic directional nanoassembly (red curve), as investigated by dark-field microscope (DFM)-SERS (λ_laser = 633 nm). The characteristic SERS peaks from A and T are indicated.