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. 2020 May 22;21(10):3661. doi: 10.3390/ijms21103661

Table 5.

Chromatographic conditions used for purification of the crude products by preparative LC.

Product Stationary Phase Mobile Phase A Mobile Phase B %B
2 Ala RP-C18 100 mM NH4HCO3 Methanol 12.5
3 Phe RP-C18 100 mM NH4HCO3 Methanol 45.0
4 Tyr RP-C18 100 mM NH4HCO3 Methanol 25.0
5 Trp RP-C18 100 mM NH4HCO3 Methanol 40.0
6 Asn RP-C18 50 mM NH4HCO3 Methanol 5.0
7 Gln RP-C18 100 mM NH4HCO3 Methanol 7.5
8 Ser RP-C18 50 mM NH4HCO3 Methanol 5.0
9 Thr RP-C18 100 mM NH4HCO3 Methanol 10.0
10 Asp HILIC 100 mM NH4HCO3 Acetonitrile 72.5
11 Glu HILIC 100 mM NH4HCO3 Acetonitrile 75.0