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. 2020 May 21;21(10):3632. doi: 10.3390/ijms21103632

Figure 2.

Figure 2

Reactivity of hybridoma culture supernatants against Gb4Cer. Hybridoma cells prepared from a Gb4Cer-VLCFA-immunized mouse were seeded into five 96-well microplates to grow as a single colony per well, and the reactivity of the antibodies in the supernatant of each well against Gb4Cer was analyzed by ELISA using horseradish peroxidase-labeled anti-mouse Immunoglobulin G (H + L). A through H and 1 through 12 indicate the row and well numbers of the 96-well plate. These results are summarized in Table 1. Arrows and arrowheads correspond to the clones analyzed in Figure 3. Arrows correspond to the clones established as hybridomas as follows: 4G5, PA5; 3H12, PA4.2; 2H4, PA7.