Figure 3.

Epitope specificity of antibodies in hybridoma culture supernatants. The reactivity against Gb4Cer, globotriaosylceramide (Gb3Cer), and lactosylceramide (LacCer) of antibodies in hybridoma culture supernatants from 27 clones showing strong reactivity to Gb4Cer in Figure 2 was analyzed by ELISA. These results are summarized in Table 2. Red and blue arrowheads correspond to clones producing anti-Gb4Cer and anti-Gb4Cer/Gb3Cer antibodies, respectively.