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. 2020 Jun 8;15(6):e0234001. doi: 10.1371/journal.pone.0234001

Fig 1. BBB impairment seen as early as 3 days into cuprizone administration.

Fig 1

(A) Fluorescent images of vessels stained with Evans blue showing increased extravasation of the dye during the first 2 weeks of cuprizone treatment. 200x mag, Scale bar = 200 μm. (B) Intensity measures showed highest levels in vessel permeability at 3 days with significant differences measured at all time points (mean ± s.e.m.). n = 6, Asterisk denotes significance (p = < 0.05) (C) Western Blot analysis of corpus callosum. Occludin at 3 days of cuprizone administration. Representative blots of Occludin and GAPDH in mice after 3 days of cuprizone administration. (D) Densitometry of western blot shows significant decrease in Occludin levels compared to controls. Data are expressed as a percentage of control with the highest control set at 100. n = 3. Asterisk denotes significance (p = < 0.05).