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. 2020 Jun 4;8(1):e000253. doi: 10.1136/jitc-2019-000253

Figure 6.

Figure 6

Macrophages mediated the antitumor effect of miR-340 against CD47 in vivo. C57BL/6 mice bearing Panc02 cells with miR-NC and miR-340 overexpression were injected intravenously with either PBS liposomes or clodronate liposomes at the indicated times (n=7 per group). (A) Schematic diagram of the macrophage depletion strategy. Immune cells isolated from tumors (B–C) and splenocytes (D–E) were stained with antibodies against CD45, CD11b and F4/80 for analysis by flow cytometry. The frequency of macrophages (CD11b+F4/80+) was expressed as a percentage of all CD45+ cells in each plot. (F) Representative images of tumors, (G) final tumor volumes and (H) final tumor weights in mice bearing Panc02 cells with miR-NC and miR-340 overexpression with or without macrophage depletion. (I) Schematic of the molecular mechanism by which miR-340 enhanced antitumor immunity. The error bars were shown as mean±SEM and the data were analyzed by two-tailed, unpaired t-test. *P<0.05; **p<0.01 and ***p<0.001.