Figure 1. Effect of Sutent monotherapy on tumor growth, MVD and the tumor microenvironment. (A) SU tumors had 1.5 days growth delay compared to size-matched VE tumors, at day-7. (N=5 in vehicle group; N=8 in Sutent-treated group). (B) Reconstituted fluorescent images of whole tumors in pre-treated, vehicle-treated group (VE) and Sutent-treated group (SU). (red:CD31. Green: pimonidazole; bar: 2 mm) (C) Representative fluorescent images of CD31 and PIMO staining in VE and SU-treated tumors. Avascular chronically hypoxic region is observed in both SU2D and SU9D tumors. (red: CD31, green: PIMO, star: central necrotic region; bar=100 μm) (D) Quantitative analysis from reconstituted whole tumor sections to reflect changes in MVD, necrosis, acute hypoxic and chronically hypoxic regions in size-matched tumors (N=5 for all groups).