Figure 3. Increase of ROS level in CD133+ Cal72 cells by wogonin. A and B: 2,7-Dichloro-dihydro-fluorescein diacetate (DCF-DA) assays were performed to determine the effect of wogonin on the ROS level. C: The effects of wogonin on the expression of peroxiredoxins PRX2, PRX3 and PRX5 proteins were detected by western blotting. D: The effects of wogonin on proteins involved in reactive oxygen species (ROS)-dependent signaling in CD133+ Cal72 cells were detected by western blotting. AKT: Protein kinase B; p-AKT: phospho-AKT; ERK: extracellular signalregulated kinase; p-ERK: ERK; STAT3: signal transducer and activator of transcription 3, p-STAT3: phospho-STAT3.