Table 2. Rab5 domains can be reconstituted in vitro.

EE MCBs were incubated for 15 min at 23 °C with 10 nM GFP-Rab5/GDI and supplemented with 1 μM GDI, 100 nM Rabex5/Rabaptin5-RFP and 1 mM GDP or GTP.

	10 nM GFP-Rab5/GDI	10 nM GFP-Rab5/GDI, 100 nM Rabex5/Rabaptin5, 1 µM GDI, 1 mM GDP	10 nM GFP-Rab5/GDI, 100 nM Rabex5/Rabaptin5, 1 µM GDI, 1 mM GTP
# Domains	0	0	449
# Beads	30	44	96
Mean # Domains/Bead	0	0	4.7
Mean intensity/Bead (a.u.)	212.31 ± 67.04	128.40 ± 4.91	447.96 ± 403.41
Mean Standard Deviation/Bead	46.70 ± 21.26	0.36 ± 0.04	237.24 ± 225.54
Mean Intensity/Domain	-	-	1326.95 ± 1026.96
Mean Intensity/Outside	212.31 ± 67.04	128.40 ± 4.91	454.63 ± 364.79
Mean domain area, µm2	-	-	1.74${\displaystyle {}_{-1.00}^{+4.74}}$
Mean domain diameter, µm	-	-	1.32