. 2020 Jun 8;9:e54434. doi: 10.7554/eLife.54434

Table 4. Rab5 domain formation in vitro is influenced by membrane composition.

MCBs with EE and PC/PS lipid composition containing 1 mol% PI(3)P and MCBs with EE and PC/PS lipid composition containing 0 mol% PI(3)P were incubated with 10 nM GFP-Rab5/GDI, 1 μM GDI, 100 nM Rabex5/Rabaptin5-RFP and 1 mM GTP for 15 min at 23 °C.

Table 4—source data 1. Rab5 domain formation in vitro is influenced by membrane composition.

elife-54434-table4-data1.xlsx^{(18.2KB, xlsx)}

	PC/PS (0% PI(3)P)	PC/PS (1% PI(3)P)	EE (0% PI(3)P)	EE (1% PI(3)P)
# Domains	0	0	13	164
# Beads	33	38	24	40
Mean # Domains/Bead	0	0	0.54	4.1
Mean intensity/Bead (a.u.)	135.48 ± 14.69	129.54 ± 11.79	140.88 ± 39.73	429.23 ± 217.66
Mean Standard Deviation/Bead	16.69 ± 8.60	13.23 ± 6.02	26.05 ± 25.50	245.40 ± 120.62
Mean Intensity/Domain	-	-	508.32 ± 143.37	1269.32 ± 556.54
Mean Intensity/Outside	135.48 ± 14.69	129.54 ± 11.79	138.59 ± 32.05	393.35 ± 194.66
Mean domain area, µm²	-	-	2.12 $_{- 1.21}^{+ 4.76}$	1.42 $_{- 0.73}^{+ 2.67}$
Mean domain diameter, µm	-	-	1.46	1.19