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. 2020 Jun 8;9:e54995. doi: 10.7554/eLife.54995

Figure 1. Identification of human MARF1 target mRNAs.

(A) Schematic diagram of full-length MARF1. (B) Distribution of crosslinked sequence reads. (C) Venn diagram illustrating the relationship of MARF1 target mRNAs identified by iCLIP in HEK293 cells with transcripts that were upregulated in Marf1-/- and Marf1D272A/D272A and germinal vesicle-stage mouse oocytes as compared to wild-type. (D) A partial list of mRNAs identified by iCLIP that were upregulated in both Marf1-/- and Marf1D272A/D272A and germinal vesicle-stage mouse oocytes as compared to wild-type.

Figure 1.

Figure 1—figure supplement 1. Representative FLAG-MARF1ΔNYN iCLIP coverage at the loci of ATXN7L3, IGFP2BP1, MAML1, FAF2 and PGAM2 loci shows specificity for the 3′UTR.

Figure 1—figure supplement 1.

The negative control iCLIP from HEK293 cells not expressing FLAG-MARF1ΔNYN shows negligible background.