Figure 4. Inhibition of nuclear export triggers Zα-dependent ZBP1-mediated cell death.

a, e, Cell death analysed by YOYO-1 uptake in MEFs (a) or LFs (e) from mice with the indicated genotypes treated with IFNγ (1,000 u ml⁻¹) (24 h pre-treatment) and LMB (1 ng ml⁻¹ for MEFs and 5 ng ml⁻¹ for LFs). b, c, d, Immunoblot analysis of total lysates from MEFs (b) or LFs (d) with the indicated genotypes and of nuclear and cytosolic extracts from WT MEFs (c) treated with combinations of IFNγ (1,000 u ml⁻¹) (24 h pre-treatment) and LMB (1 ng ml⁻¹ for MEFs and 5 ng ml⁻¹ for LFs). Data information: data are representative of four (a), two (b, d) or five (c, e) independent experiments. Graphs in a and e show mean of technical triplicates (n = 3). GAPDH and histone 3 (H3) were used as loading controls and cytosolic and nuclear fraction markers respectively for immunoblot analysis. For gel source data, see Supplementary Fig. 1.