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. Author manuscript; available in PMC: 2020 Sep 25.
Published in final edited form as: Nature. 2020 Mar 25;580(7803):391–395. doi: 10.1038/s41586-020-2129-8

Extended Data Figure 2. ZBP1 exhibits Zα-dependent and -independent functions in anti-viral defence.

Extended Data Figure 2

a, Cell death analysed by measuring LDH release in supernatants of LFs with the indicated genotypes infected with IAV (MOI:1 or 5) for 24 h. Data show mean from technical triplicates (n = 3). b, Immunoblot analysis of total lysates from LFs with the indicated genotypes infected with IAV (MOI:5) for 8 h with the indicated antibodies. GAPDH was used as a loading control. c-e, Viral titres in spleen (c), liver (d) or salivary gland (e) from mice with the indicated genotypes 5 days (c, d) or 14 days (e) after intraperitoneal inoculation with 1 × 106 MCMV-M45mutRHIM. Graphs show values from individual mice as well as mean ± s.e.m. P values by two-sided nonparametric Mann–Whitney test. The dotted horizontal red line depicts the detection limit of the assay. Data information: data shown are representative of two independent experiments. For gel source data, see Supplementary Fig. 1.