Skip to main content
. Author manuscript; available in PMC: 2020 Jun 8.
Published in final edited form as: Biochim Biophys Acta Mol Cell Biol Lipids. 2020 May 1;1865(8):158730. doi: 10.1016/j.bbalip.2020.158730

Fig. 6. Neutral lipid stores of primary murine WT and LAL-ko HSCs are increased upon lipid loading.

Fig. 6

Primary murine WT and LAL-deficient HSCs (= LAL-ko) were isolated by selective detachment for 14 days in DMEM containing 10% FCS and ROH (5 μM). Cells were plated in 8-well chamber slides. The next day, media were changed to DMEM containing 10% FCS (= basal) or DMEM containing 10% FCS, ROH (20 μM), and oleic acid (100 μM) (= loaded). Cells were further incubated overnight. Images of CARS signal (Ex. 816.7 nm + 1064 nm) and autofluorescence (Ex. 816.7 nm) were recorded and merged. Insets depict selected areas of the images. Transmissions images visualize cell structures. Scale bar = 5 μm.